Recombinant TGF beta 1 (Transforming growth factor beta 1), Swine, AF

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Recombinant TGF beta 1 (Transforming growth factor beta 1), Swine, AF

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SDS- PAGE analysis of recombinant swine TGF beta 1

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产品简介

Catalog No.

PY2922

Source

Swine Cytokines

UniProt ID

P07200

Reactivity

Measure by its ability to inhibit IL-4-induce proliferation in HT-2 cells. The ED50 for this effect is <0.1 ng/mL.

Application

Cell culture, Elisa

Calculated Mol Weight

14 kDa

Observed Mol Weight

11-17 kDa

Recommended Dilution

It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved. In some experiments, it recommends to add 10 mM HCl when reconstitute lyophilized protein.

Alternative Names

TGF-BETA-1, TGFB1

Host

Escherichia coli

Isotype

Clonality

Modification

His-tag at the C-terminus

Immunogen

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Purification

>98% as determined by SDS-PAGE. Ni-NTA chromatography

Concentration

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Buffer System

<0.1 EU per 1 μg of the protein by the LAL method. The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 4.5.

Storage Condition

Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months.

Description

Transforming growth factor beta 1 or TGF-β1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, and apoptosis. In humans, TGF-β1 is encoded by the TGFB1 gene.

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PY2922

规格：

5μg, 20μg

价格：

¥1500/5μg, ¥4000/20μg

货期：

3-4天

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