Recombinant IL-10 (Interleukin-10), Mouse, AF - 上海埃必威生物技术有限公司

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Recombinant IL-10 (Interleukin-10), Mouse, AF

Recombinant IL-10 (Interleukin-10), Mouse, AF

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SDS- PAGE analysis of recombinant mouse IL-10

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产品简介

Catalog No.

PY2543

Source

Mouse Cytokines

UniProt ID

P18893

Reactivity

Measure by its ability to induce MC/9‑2 cells proliferation. The ED50 for this effect is <1 ng/mL. The specific activity of recombinant mouse IL-10 is > 1 x 106 IU/mg.

Application

Cell culture, Elisa

Calculated Mol Weight

20 kDa

Observed Mol Weight

19 kDa

Recommended Dilution

It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

Alternative Names

B-TCGF, CSIF, TGIF

Host

Escherichia coli

Isotype

Clonality

Modification

His-tag at the C-terminus

Immunogen

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Purification

>98% as determined by SDS-PAGE. Ni-NTA chromatography

Concentration

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Buffer System

<0.1 EU per 1 μg of the protein by the LAL method. The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4.

Storage Condition

Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months.

Description

Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans, interleukin 10 is encoded by the IL10 gene. IL-10 signals through a receptor complex consisting of two IL-10 receptor-1 and two IL-10 receptor-2 proteins. Consequently, the functional receptor consists of four IL-10 receptor molecules. IL-10 binding induces STAT3 signalling via the phosphorylation of the cytoplasmic tails of IL-10 receptor 1 + IL-10 receptor 2 by JAK1 and Tyk2 respectively.

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PY2543

规格：

5μg, 20μg, 100μg, 500μg, 1mg

价格：

¥800/5μg, ¥2000/20μg, ¥6000/100μg, ¥18000/500μg, ¥26000/1mg

货期：

3-4天

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