Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1,α skeletal muscle actin Monoclonal Antibody was diluted at 1:100(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.