Western blot analysis of extracts from CHO-K1,CHO-K1 transfected by Flag-EpCAM, MCF7(EpCAM positive),HCT116(EpCAM positive),and Hela(EpCAM negative) cell lysates using EpCAM mouse mAb (1:1000 diluted).Predicted band size:39KDa.Observed band size:39/35KDa.