alpha Tubulin (4C9) Mouse mAb_单克隆抗体-上海泊湾生物技术有限公司

alpha Tubulin (4C9) Mouse mAb

Source: Mouse mAb Catalog No. : AB3445

alpha Tubulin (4C9) Mouse mAb (AB3445)
UniProt:P68363
Application:WB,IHC,ICC/IF,IP
Reactivity:Human,Mouse,Rat
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3445

 Datasheet

数据表

UniProt

P68363
Mol Weight

52kDa
Alternative Names

alpha tubulin 2, B alpha 1, H2 alpha, Hum a tub 1, TUBA1, TUBA1A,Tubulin alpha 1chain
Immunogen

Recombinant Protein of Tubulin alpha-1A chain
Storage

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Mouse IgG1
Clonality

Monoclonal
Description

Swiss-Prot Acc.P68363.Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.SitesFeature keyPosition(s)Description

Applications

Reactivity

Human,Mouse,Rat
Application

WB,IHC,ICC/IF,IP
Recommended dilution

WB: 1/1000-1/5000 IHC: 1/100-1/500 ICC/IF: 1/50-1/200 IP: 1/20

Western blot detection of alpha Tubulin in A549,K562,THP-1,Rat Brain,Hela cell lysates using alpha Tubulin (4C9) Mouse mAb(1:5000 diluted).Predicted band size:52KDa.Observed band size:52KDa.
Immunohistochemical analysis of paraffin-embedded human Colon caricnoma using alpha tubulin mouse mAb diluted at 1:500.
IF analysis of Hela with (Left) and DAPI (Right) diluted at 1:100.
1) Input: Mouse Brain Tissue Lysate 2) IP product: IP dilute 1:20
Western blot detection of alpha Tubulin in MCF7,3T3,Rat Brain,Jurkat and Hela cell lysates using alpha tubulin mouse mAb (1:20000 diluted).Predicted band size:52kDa.Observed band size:52kDa.