Histone H3 (acetyl K14) Antibody
Source: Rabbit mAb Catalog No. : CY5272
Histone H3 (acetyl K14) Antibody (CY5272)
UniProt:P68431
Application:WB,IHC,ICC/IF,IP
Reactivity:Human,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY5272
数据表
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UniProt
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Mol Weight15kDa
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Alternative NamesH3 histone family, member A; H3/A; H31; H3FA; H3FB; H3FC; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; histone 1, H3a; histone cluster 1, H3a; Histone H3.1;
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ImmunogenA synthesized peptide derived from human Histone H3 (acetyl K14)
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionH3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers.
Applications
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ReactivityHuman,Rat
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ApplicationWB,IHC,ICC/IF,IP
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Recommended dilutionWB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50
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All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.
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Western blot analysis of Acetyl-Histone H3 (K14) expression in C6 cell lysate.
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Immunofluorescent analysis using the Antibody at 1:25 dilution.
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Immunofluorescent analysis using the Antibody at 1:25 dilution.
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Immunofluorescent analysis using the Antibody at 1:25 dilution.