Alpha Skeletal Muscle Actin (2B10) Mouse mAb
Source: Mouse mAb Catalog No. : AB3453
Alpha Skeletal Muscle Actin (2B10) Mouse mAb (AB3453)
UniProt:P68133
Application:WB,IHC,ICC/IF,IP
Reactivity:Human,Mouse,Rat
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3453
数据表
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UniProt
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Mol Weight42kDa
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Alternative NamesACTA, ACTA1, ACTC, ACTC1, Actin alpha cardiac muscle, ACTS, Alph
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ImmunogenSynthetic Peptide of α skeletal muscle actin
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG
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ClonalityMonoclonal
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DescriptionSwiss-Prot Acc.P68133.Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.MiscellaneousIn vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF,IP
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Recommended dilutionWB: 1/1000-1/10000 IHC: 1/50-1/200 ICC/IF: 1/50-1/200 IP: 1/50
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Western blot detection of Alpha Skeletal Muscle Actin in T47D,3T3,COS7,C6 and Hela cell lysates using Alpha Skeletal Muscle Actin mouse mAb (1:10000 diluted).Predicted band size:42KDa.Observed band size:42KDa. -
Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1,α skeletal muscle actin Monoclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted
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