Histone H3 (6G2) Mouse mAb
Source: Mouse mAb Catalog No. : AB0062
Histone H3 (6G2) Mouse mAb (AB0062)
UniProt:P68431
Application:WB,IHC,ICC/IF,IP
Reactivity:Human,Mouse,Rat,Yeast
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB0062
数据表
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UniProt
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Mol Weight15kDa
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Alternative NamesH3 histone; HIST1H3A; Histone cluster 1; H3a
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ImmunogenRecombinant Protein of Histone H3
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG1
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ClonalityMonoclonal
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DescriptionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.MiscellaneousThis histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).
Applications
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ReactivityHuman,Mouse,Rat,Yeast
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ApplicationWB,IHC,ICC/IF,IP
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Recommended dilutionWB: 1/1000-1/10000 IHC: 1/50-1/200 ICC/IF: 1/100-1/200 IP: 1/20
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Western blot analysis of Histone H3 (6G2) in C6,3T3 and MCF7 lysates using Histon H3 antibody.
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Immunohistochemistry analysis of paraffinembedded Rattestis tissue using Histone H3 antibody.
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Immunofluorescence analysis of Histone H3 in Hela using Histone H3 antibody(Right)and DAPI(Left).
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Immunoprecipitation analysis of Histone H3 (6G2) in Hela lysates using Histone H3 (6G2) antibody.
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Western blot analysis of Histone H3 in C6,3T3,Hela lysates using Histone H3 (6G2) antibody.