PCNA (9C9) Mouse mAb
Source: Mouse mAb Catalog No. : AB0060
PCNA (9C9) Mouse mAb (AB0060)
UniProt:P12004
Application:WB,IHC
Reactivity:Human,Mouse,Rat
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB0060
数据表
-
UniProt
-
Mol Weight29kDa
-
Alternative NamesProliferating Cell Nuclear Antigen; DNA polymerase delta auxiliary protein; PCNAR
-
ImmunogenSynthetic Peptide of PCNA
-
StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
-
PurificationAffinity-chromatography
-
IsotypeMouse IgG1
-
ClonalityMonoclonal
-
DescriptionAuxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
Applications
-
ReactivityHuman,Mouse,Rat
-
ApplicationWB,IHC
-
Recommended dilutionWB: 1/1000-1/10000 IHC: 1/50-1/200
-
Western blot analysis of PCNA in T47D,3T3 and COS7 lysates using PCNA antibody.
-
Western blot analysis of PCNA in Jurkat,CHOK1,3T3,Hela lysates using PCNA (9C9) antibody.
-
Western blot analysis of PCNA (9C9) in MOT4,Raw264.7,293T,CEM lysates using PCNA (9C9) antibody.
-
Immunohistochemistry analysis of paraffinembedded Human breast cancer tissue using PCNA (9C9) antibody.