PCNA Antibody
Source: Rabbit mAb Catalog No. : AB0051
PCNA Antibody (AB0051)
UniProt:P12004
Application:WB,IHC,ICC/IF,IP,FC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAb
References(16)
http://www.abways.cn/showproduct.asp?cid=AB0051
数据表
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UniProt
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Mol Weight29kDa
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Alternative NamesCyclin; DNA polymerase delta auxiliary protein; MGC8367; PCNA; Proliferating cell nuclear antigen
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ImmunogenA synthesized peptide derived from human PCNA
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionPCNA This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Belongs to the PCNA family. Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF,IP,FC
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Recommended dilutionWB 1:3000~1:10000 IHC 1:50~1:200 ICC/IF 1:100~1:500 IP 1:50~1:100 FC 1:200~1:500
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Western blot analysis of PCNA expression in (1) Hela cell lysate; (2) HepG2 whole cell lysate; (3) U937 whole cell lysate; (4) Mouse spleen lysate with PCNA Antibody.
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Immunohistochemical analysis of paraffin-embedded human colon, using PCNA Antibody.
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Immunofluorescent analysis of Hela cells, using PCNA Antibody.
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Prostaglandin E1 Inhibited Diabetes-Induced Phenotypic Switching of Vascular Smooth Muscle Cells Through Activating Autophagy. -Cellular Physiology and Biochemistry
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CircDLST promotes the tumorigenesis and metastasis of gastric cancer by sponging miR-502-5p and activating the NRAS/MEK1/ERK1/2 signaling. -Molecular Cancer
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All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1w dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1w dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1w dilution for 1 hour at room temperature.
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Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution)
引用文献(16条)
Cytidine monophosphate kinase is inhibited by the TGF-β signalling pathway through the upregulation of miR-130b-3p in human epithelial ovarian cancer.-Cellular Signalling
Prostaglandin E1 Inhibited Diabetes-Induced Phenotypic Switching of Vascular Smooth Muscle Cells Through Activating Autophagy.-Cellular Physiology and Biochemistry
CircDLST promotes the tumorigenesis and metastasis of gastric cancer by sponging miR-502-5p and activating the NRAS/MEK1/ERK1/2 signaling.-Molecular Cancer
Contribution of acid sphingomyelinase to angiotensin II-induced vascular adventitial remodeling via membrane rafts/Nox2 signal pathway.-Life Sciences
Inhibition of neointima hyperplasia by the combined therapy of linagliptin and metformin via AMPK/Nox4 signaling in diabetic rats-Free Radical Biology and Medicine