GAPDH Mouse Monoclonal Antibody
Source: Mouse mAb Catalog No. : AB0038
GAPDH Mouse Monoclonal Antibody (AB0038)
UniProt:P04406
Application:WB,IHC,ICC/IF,IP,FC
Reactivity:Human,Mouse,Rat,Mk,Ch,Ze,Fish
Source:Mouse mAb
References(3)
http://www.abways.cn/showproduct.asp?cid=AB0038
数据表
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UniProt
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Mol Weight36kDa
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Alternative Namesaging-associated gene 9 protein; G3P; G3PD; GAPD; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; MGC88685;
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ImmunogenA synthesized peptide derived from human GAPDH Mouse Monoclonal
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG
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ClonalityMonoclonal
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DescriptionGlyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.
Applications
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ReactivityHuman,Mouse,Rat,Mk,Ch,Ze,Fish
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ApplicationWB,IHC,ICC/IF,IP,FC
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Recommended dilutionWB 1:5000~1:20000 IHC 1:100~1:500 ICC/IF 1:100~1:250 IP 1:50 FC 1:50
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Western blot analysis of GAPDH expression in (1) Hela cell lysate; (2)Jurkat cell lysate; (3)Mouse kidney lysate; (4) Mouse spleen lysate; (5) RAW 264.7 cell lysate; (6) Rat brain lysate with GAPDH Mouse Monoclonal Antibody.
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Immunohistochemical analysis of paraffin-embedded human colon cancer, using GAPDH Mouse Monoclonal Antibody.
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Immunofluorescent analysis of Hela cells, using GAPDH Mouse Monoclonal Antibody .
引用文献(3条)
FGF2 disruption enhances thermogenesis in brown and beige fat to protect against adiposity and hepatic steatosis-Molecular Metabolism (MOL METAB)
Peripheral IL-6/STAT3 signaling promotes beiging of white fat-BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH (BBA - Molecular Cell Research)
AGGF1 therapy inhibits thoracic aortic aneurysms by enhancing integrin α7-mediated inhibition of TGF-β1 maturation and ERK1/2 signaling -nature communications