beta Actin Antibody
Source: Rabbit mAb  Catalog No. : AB0035  
beta Actin Antibody (AB0035)
UniProt:P60709
Application:WB,IHC,ICC/IF
Reactivity:Human,Mouse,Rat
Source:Rabbit mAb
References（99）
http://www.abways.cn/showproduct.asp?cid=AB0035
数据表
  • UniProt
    P60709
  • Mol Weight
    42kDa
  • Alternative Names
    ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta;
  • Immunogen
    A synthesized peptide derived from human beta Actin
  • Storage
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Purification
    Affinity-chromatography
  • Isotype
    Rabbit IgG
  • Clonality
    Monoclonal
  • Description
    beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.
Applications
  • Reactivity
    Human,Mouse,Rat
  • Application
    WB,IHC,ICC/IF
  • Recommended dilution
    WB 1:5000~1:50000 IHC 1:50~1:200 ICC/IF 1:50~1:200
  • AB0035
    Immunofluorescent analysis using the Antibody at 1:50 dilution.
  • AB0035
    Western blot analysis of beta Actin expression in (1)HeLa cell lysate; (2)Human fetal kidney lysate; (3) NIH/3T3 cell lysate; (4) PC-12 cell lysate.
  • AB0035
    All lanes use AB0035 beta Actin Antibody at 1:40000 dilution for 1 hour at room temperature.
  • AB0035
    All lanes use AB0035 beta Actin Antibody at 1:50000 dilution for 1 hour at room temperature.
  • AB0035
    Schisandrin B inhibits cell proliferation and induces apoptosis in human cholangiocarcinoma cells. -Oncology Reports
  • AB0035
    Chloride intracellular channel 1 regulates the antineoplastic effects of metformin in gallbladder cancer cells. -cancer science
  • AB0035
    JLX001 Modulated the Inflammatory Reaction and Oxidative Stress in pMCAO Rats via Inhibiting the TLR2/4-NF-κB Signaling Pathway. -Neurochemical Research
引用文献（99条）
Schisandrin B inhibits cell proliferation and induces apoptosis in human cholangiocarcinoma cells.-Oncology Reports
Chloride intracellular channel 1 regulates the antineoplastic effects of metformin in gallbladder cancer cells.-cancer science
Improvement of vascular dysfunction by argirein through inhibiting endothelial cell apoptosis associated with ET-1/Nox4 signal pathway in diabetic rats.-Scientific Reports
Histone methyltransferase SETD2 regulates osteosarcoma cell growth and chemosensitivity by suppressing Wnt/β-catenin signaling.-Biochemical and Biophysical Research Communications
Naringin Dihydrochalcone Ameliorates Cognitive Deficits and Neuropathology in APP/PS1 Transgenic Mice.-Front Aging Neurosci
Catalog No:AB0035
  • 规格：50μl, 100μl
  • 价格：¥600/50μl, ¥1000/100μl
  • 货期：现货
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