Alternative Names:

GFP ; yfp ; Green fluorescent protein ;

Application:

WB IHC ICC/IF IP

Reactivity:

All

Dilution:

WB 1:3000~1:10000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:100

	Western blot analysis of GFP protein expression in (1) 293T cell lysate; (2) 293T cell transfected with GFP protein lysate.
	Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histoneto-Protamine Exchange during Spermiogenesis. -CELL

Purification:

Affinity-chromatography

Specificity:

GFP-Tag Mouse Monoclonal antibody detects endogenous levels of C-terminal, internal, and N-terminal GFP-tagged proteins.

Immunogen:

Full-length GFP protein

Description:

Green fluorescent protein(GFP) has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -23°C long term. Avoid freeze / thaw cycle.